East End Arts Announces Open Call for Street Painting Artists, Fine ...

East End Arts? 17th Annual Community Mosaic Street Painting Festival: May 26, 2013

(RIVERHEAD, NY) ?? East End Arts is pleased to announce an open call for artist participation at the 17th Annual Community Mosaic Street Painting Festival to be held Sunday, May 26, 2013, from 12PM to 5PM in downtown Riverhead, NY. This beloved annual fun-for-the-whole-family festival includes street painting on East Main Street, fine arts & crafts market of local artists and artisans, live music and entertainment, family activities, face painting, a drum circle, dance, gourmet food trucks, and more. This call is open to all artists to exhibit and sell their work in the fine arts & crafts market, as well as for artists to participate in the street painting activities. This event draws over 5,000 local residents and tourists to downtown Riverhead for this Memorial Day Weekend tradition where families and friends, artists, musicians, and lovers of the arts, young and old, enjoy the creative spirit together. All activities take place in downtown Riverhead on East Main Street, between Roanoke Ave and East Street, and on the grounds of East End Arts (133 and 141 East Main Street.) This event is sponsored by Bridgehampton National Bank.

More Information for Fine Artisan & Crafts Vendors

East End Arts invites professional artists and craftspeople to submit photos of their original work for consideration for the fine art and craft sale held outdoors. Arts and crafts of fine quality that are priced for a family event are recommended. Artisans of homemade goods such as soaps, potpourri, jams, etc. are encouraged to display and sell their wares. Artists and artisans may reserve a 10? x 10? space for a $50 for EEA members or $150 for non-members. Display setup, which begins at 9AM the day of the event, and removing the artwork (no earlier than 5PM), will be the responsibility of the artists. Popup tents, tables and chairs will not be provided. All sales transactions are between the artist and purchaser and the artists are responsible for sales-tax permits. There are no commissions taken by East End Arts on sales of artwork and crafts. Registration deadline: May 15, 2013. For an application, please visit http://eastendarts.org/Temp2013/MosaicArtistApp.pdf.

More Information for Street Painting Artists

?I Madonnari? is a street painting art form dating back to 16th Century Italy, where street painters created works of art in front of cathedrals to pay homage to the Virgin Mary. Street painters that are over the age of 14 are encouraged to register in advance. Pre-registered street painters will be matched with a sponsored square on a first-come-first-serve basis. Chalk and blending materials are provided. It is recommended that street painters bring kneepads and a hat. Artwork must be suitable for public viewing. No words or symbols intended as advertising may appear within the image, however artists may sign their names. If the square is sponsored, the sponsor?s name will appear above the space. Street painting squares are given on a first-come basis. Street painters who pre-register will be notified of acceptance prior to the event. Street painters agree that work done in conjunction with this festival may be reproduced by photograph or video and used by the East End Arts for promotional purposes. Registration deadline: May 15, 2013. For registration form please visit http://eastendarts.org/Temp2013/CM2013StreetPaintingApp.pdf.

For more information contact Kristina Gosline, Event Coordinator, at 631-727-0900 x302 or events@eastendarts.org, or stop by the East End Arts Gallery located at 133 East Main Street in Riverhead to sign-up.

East End Arts, established in 1972, is a 501(c)3 not-for-profit arts organization, and operates a School of the Arts, an ArtGallery, and presents a variety of events and programs to promote the arts year-round. Proceeds from all events, sales, classes, donations, and memberships benefit the mission and commitment to building and enriching our community through the arts by way of education, support, advocacy, and inspiration.

Source: http://www.longislandexchange.com/press/2013/04/03/east-end-arts-announces-open-call-for-street-painting-artists-fine-artisan-and-crafts-vendors/

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Crucial step in human DNA replication observed for the first time

Tuesday, April 2, 2013

For the first time, an elusive step in the process of human DNA replication has been demystified by scientists at Penn State University. According to senior author Stephen J. Benkovic, an Evan Pugh Professor of Chemistry and Holder of the Eberly Family Chair in Chemistry at Penn State, the scientists "discovered how a key step in human DNA replication is performed." The results of the research will be published in the journal eLife on 2 April 2013.

Part of the DNA replication process -- in humans and in other life forms -- involves loading of molecular structures called sliding clamps onto DNA. This crucial step in DNA replication had remained somewhat mysterious and had not been well studied in human DNA replication. Mark Hedglin, a post-doctoral researcher in Penn State's Department of Chemistry and a member of Benkovic's team, explained that the sliding clamp is a ring-shaped protein that acts to encircle the DNA strand, latching around it like a watch band. The sliding clamp then serves to anchor special enzymes called polymerases to the DNA, ensuring efficient copying of the genetic material. "Without a sliding clamp, polymerases can copy very few bases -- the molecular 'letters' that make up the code of DNA -- at a time. But the clamp helps the polymerase to stay in place, allowing it to copy thousands of bases before being removed from the strand of DNA," Hedglin said.

Hedglin explained that, due to the closed circular structure of sliding clamps, another necessary step in DNA replication is the presence of a "clamp loader," which acts to latch and unlatch the sliding clamps at key stages during the process. "The big unknown has always been how the sliding clamp and the clamp loader interact and the timing of latching and unlatching of the clamp from the DNA," said Hedglin. "We know that polymerases and clamp loaders can't bind the sliding clamp at the same time, so the hypothesis was that clamp loaders latched sliding clamps onto DNA, then left for some time during DNA replication, returning only to unlatch the clamps after the polymerase left so they could be recycled for further use."

To test this hypothesis, the team of researchers used a method called F?rster resonance energy transfer (FRET), a technique of attaching fluorescent "tags" to human proteins and sections of DNA in order to monitor the interactions between them. "With these tags in place, we then observed the formation of holoenzymes -- the active form of the polymerase involved in DNA replication, which consists of the polymerase itself along with any accessory factors that optimize its activity," Hedglin said. "We found that whenever a sliding clamp is loaded onto a DNA template in the absence of polymerase, the clamp loader quickly removed the clamp so that free clamps did not build up on the DNA. However, whenever a polymerase was present, it captured the sliding clamp and the clamp loader then dissociated from the DNA strand."

The team members also found that, during the moments when both the clamp loader and the clamp were bound to the DNA, they were not intimately engaged with each other. Rather, the clamp loader released the closed clamp onto the DNA, allowing an opportunity for the polymerase to capture the clamp, completing the assembly of the holoenzyme. Subsequently, the clamp loader dissociated from DNA. "Our research demonstrates that the DNA polymerase holoenzyme in humans consists of only a clamp and a DNA polymerase. The clamp loader is not part of it. It disengages from the DNA after the polymerase binds the clamp," Hedglin added.

Benkovic noted that this mechanism provides a means for the cell to recycle scarce clamps when they are not in use for productive replication.

###

Penn State: http://live.psu.edu

Thanks to Penn State for this article.

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Source: http://www.labspaces.net/127551/Crucial_step_in_human_DNA_replication_observed_for_the_first_time

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Autopsy reveals 'Buckwild' star died of carbon monoxide poisoning

REUTERS - An autopsy on Shain Gandee, a cast member of MTV reality show "Buckwild," showed he died from carbon monoxide poisoning, Kanawha County Sheriff's Department said on Tuesday.

Deputy Brian Humphreys told Reuters that the deaths of Gandee, 21, his uncle David, 48, and friend Donald Robert Myers, 27, on Monday morning were ruled "accidental" after coroners completed autopsies on all three men.

Gandee was found dead in the driver's seat of his Ford Bronco pick-up truck in Sissonville, in Kanawha County, a rural West Virginia community.

The truck was found by one of Gandee's friends after the MTV star was reported missing by family members following a late-night session in a local bar.

Gandee's truck was partially submerged in a mud pit, and the vehicle's exhaust pipe was below the surface of the mud, the sheriff's department said in a news release.

MTV said in a statement on Monday that it was "shocked and saddened" by Gandee's death. A spokesperson told Reuters that the network had suspended production on "Buckwild."

Gandee was one of the lead cast members of "Buckwild," which follows a group of young adults living in rural West Virginia and has attracted some criticism for its portrayal of the cast's hard-partying ways.

Gandee was often seen on the show "four-wheeling" in his truck, driving through makeshift courses on off-road terrain, and "mudding," where he would drive at high speeds through mud pits.

(Reporting By Piya Sinha-Roy in Los Angeles; Editing by Eric Kelsey and Philip Barbara)

Source: http://news.yahoo.com/autopsy-shows-gandee-buckwild-died-carbon-monoxide-poisoning-210427735--finance.html

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Buzz Aldrin's Vision for Mars Exploration Detailed in New Book

Pioneering astronaut Buzz Aldrin made history as the second man to walk on the moon in 1969, just after Neil Armstrong during the historic Apollo 11 lunar landing mission. More than four decades later, he wants NASA to set its sights on more ambitious destinations, far beyond the moon. Aldrin's target: Mars.

In his upcoming book, "Mission to Mars: My Vision for Space Exploration" (National Geographic Books), Buzz Aldrin argues that NASA should strive to put humans on the Red Planet by the mid-2030s and he lays out a plan for how to make it happen.

"Do not put NASA astronauts on the moon. They have other places to go," Aldrin said in the statement.

The book will apparently delve into Aldrin's past ? including his service as an Air Force pilot during the Korean War, his initial rejection by NASA and his voyage to the moon ? but also promises a critique of current space policy, examining the economic, political and technological viability of various options to explore the solar system.

In the 1980s, Aldrin adapted his expertise in orbital rendezvous to conceptualize the "Aldrin Mars Cycler," a spacecraft transportation system perpetually cycling between Earth and Mars that would make it possible to ferry astronauts back and forth to the Red Planet.

Aldrin has co-authored more than six books and the new one, which will hit stores on May 7, was co-written with space journalist Leonard David, who is a frequent contributor to SPACE.com.

Follow SPACE.com on Twitter @Spacedotcom. We're also on Facebook and Google+.

Copyright 2013 SPACE.com, a TechMediaNetwork company. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.

Source: http://news.yahoo.com/buzz-aldrins-vision-mars-exploration-detailed-book-115030612.html

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Afghans warned: The taxman is coming after you

By Katharine Houreld

KABUL (Reuters) - One of Afghanistan's most surprising success stories lies tucked away on a potholed street notorious for suicide bombings and lined with rusting construction equipment.

The work of the country's top tax collector is more inspiring than the view from his office in Kabul. Taxes and customs raised $1.64 billion last financial year, a 14-fold increase on 10 years ago. That means, now, the government can pay just over half of its recurrent costs such as salaries.

Thanks to tougher enforcement procedures, Afghanistan's tax to GDP ratio today stands above 11 percent - ahead of neighboring Pakistan's dismal 9 percent.

Increasing revenues is vital as donors begin reducing aid ahead of the 2014 drawdown of NATO troops, who have provided the backbone for security since U.S. forces invaded after the September 11 attacks on the United States.

By the end of this year the United States alone will have spent $100 billion on Afghan reconstruction. But future pledges are a fraction of that.

"We are largely dependent on international aid. We would like to be independent," said Abdurrahman Mujahid, the new head of the revenue department. "I would like a sustainable Afghanistan for all the children."

Despite rising revenues, the government will rely heavily on donors for years to come. Taxes, customs and mining revenue will only meet $2.5 billion out of a $7 billion budget this year.

Most of the revenue comes from large corporate taxpayers, who complain their payments have not improved power cuts, potholed roads or security.

Corporations pay a flat tax of 20 percent - the same rate for an individual earning over $2,000 a month.

But unlike developed countries where personal income tax generates a sizeable chunk of revenue, most Afghans scoff at the idea of giving the government some of their meager earnings.

The average annual income, in a country ranked one of the world's poorest, is just $470, according to the World Bank. Those making less than $100 a month don't have to pay tax.

"It's not a good government," said moneychanger Abdurrahman Arif, 28, as he held a wad of soiled notes and scanned for customers. "I don't pay tax. The rich people don't and the government should go to them before they come to me."

Afghanistan has a similar problem to neighboring Pakistan - the very wealthy don't pay their share, and weak institutions often have little way of forcing them.

Authorities admit that taxing the rich isn't easy in a country where the powerful often command militias. But Mujahid promises tax evaders will "be introduced to the law enforcement agencies".

SUBSTANTIAL ACHIEVEMENT

Much of Afghanistan's money is in an undocumented black economy. Corruption is endemic and the country produces 90 percent of the world's opium. Billions of dollars in cash leave the country every year in suitcases.

The security situation is discouraging. Taliban and other militias have made gains in many areas as foreign combat forces wind down their missions.

But some Afghans still manage to make money. Many businesses are fuelled by the aid dollars that have poured into the country over the last decade. Luxury supermarkets, travel agencies and stationery shops crowd the capital's streets.

A U.S. embassy official in Kabul commended Afghanistan's ability to raise tax revenues.

"It's a pretty substantial achievement," the official said, but noted the nation still faced a large funding gap, partly because of its huge security bill.

"It's going to continue being a problem until they can get revenues from the extractive industry, and that's going to take some time," the official said, referring to Afghanistan's rich but undeveloped mineral deposits.

Donors currently pay for just under half Afghanistan's operating costs - mostly government salaries - and more than three-quarters of all development projects like roads, dams and electricity equipment.

Rampant corruption means this money is often stolen, angering donors, fuelling anti-government rage and keeping aid from some of the world's neediest families.

Donors hope that if Afghans foot more of the bill for public services they may become less tolerant of graft from their leaders.

PUGNACIOUS PREDECESSOR

Mujahid, the new head of the revenue department, has large shoes to fill. His predecessor Ahmad Shah Zamanzai oversaw much of the department's growth and didn't shrink from confrontation.

When a vice-president refused to pay tax on income from renting out houses he owned, Zamanzai threatened to leak it to the press. Elections were approaching. The vice president paid up.

Under Zamanzai, the tax department jailed more than 20 tax evaders, froze bank accounts, slapped on travel bans and shuttered the premises of businesses that refused to pay.

In one showdown, he took on the glitzy wedding halls that have mushroomed up in the capital. When the 60 or so venues refused to pay their dues, he had police padlock a dozen of the biggest until the rest fell into line.

Zamanzai was appointed head of the state-run Pashtany Bank as part of a bureaucratic reshuffle this month. His first task, he said, would be to use skills honed in the tax department to extract overdue loan repayments from powerful Afghans.

But the tough tax enforcement has angered some businessmen.

Najib Ullah Latify's spotless factory, full of humming machinery and rows of workers in blue overalls and yellow hard hats, stands a few minutes drive from the tax office. High Standard Pipe employs 850 people and supplies pipes for projects providing clean water all over Afghanistan.

Latify said he'd expand but harassment from the tax man was hurting his business.

In recent years, he says, he's been repeatedly overcharged by the tax office and promised refunds have not been credited. Officials frequently offer to slash his tax bill in return for bribes, he added. When he refuses, he says, officials disrupt his imports and suspend his license.

"I don't know what to do, I have shouted everywhere that they are ruining my business," he said.

"I don't mind paying taxes. Even if 60 percent of it is spent on drinking and shopping and trips for (politicians') wives, maybe 40 percent will go to schools or hospitals. But they must tax me correctly."

The new tax chief, Mujahid, was not familiar with Vitaly's case, but promised to investigate. More than 10 tax collectors - whose basic salaries start at $180 a month - have been fired for corruption in the last two years.

"Corruption is a part of public life in Afghanistan," said Mujahid. "We have the aim to make this department corruption-free."

This year he's planning to finish computerizing tax records, usher through a law on Value Added Tax, and strengthen collection in the provinces - more than 90 percent of government taxes currently come from the capital.

"There's a lot of achievements, but for sure we have problems, and the biggest problem is corruption," he said.

(Editing by Jeremy Laurence)

Source: http://news.yahoo.com/afghans-warned-taxman-coming-075922090.html

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'The Hobbit: The Desolation Of Smaug' Sneak Peek Now Online For All To Watch

Last week, Peter Jackson gave an exclusive sneak peek of "The Hobbit: The Desolation of Smaug" to the fans of "An Unexpected Journey" who bought the Blu-ray or DVD. During an hour-long livestream, Jackson answered some questions about the next movie, complete with behind-the-scenes footage from the film and two pieces of concept art. Always [...]

Source: http://moviesblog.mtv.com/2013/04/01/the-hobbit-the-desolation-of-smaug-sneak-peek/

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NKorea's parliament meets amid nuclear tension

SEOUL, South Korea (AP) ? After weeks of war-like rhetoric, North Korean leader Kim Jong Un gathered legislators Monday for an annual spring parliamentary session taking place one day after top party officials adopted a statement declaring building nuclear weapons and the economy the nation's top priorities.

The meeting of the Supreme People's Assembly follows near-daily threats from Pyongyang, including vows of nuclear strikes on South Korea and the U.S.

Pyongyang has reacted with anger over routine U.S.-South Korean military drills and a new round of U.N. and U.S. sanctions that followed its Feb. 12 underground nuclear test, the country's third. Analysts see a full-scale North Korean attack as unlikely and say the threats are more likely efforts to provoke softer policies toward Pyongyang from a new government in Seoul, to win diplomatic talks with Washington and to solidify the young North Korean leader's military credentials at home.

On Sunday, Kim and top party officials adopted a declaration calling nuclear weapons the "the nation's life" and an important component of its defense, an asset that wouldn't be traded even for "billions of dollars." Pyongyang cites the U.S. military presence in South Korea as a main reason behind its drive to build missiles and atomic weapons. The U.S. has stationed tens of thousands of troops in South Korea since the Korean War ended in a truce in 1953.

North Korea also has threatened in recent days to shut down a jointly run factory complex in the North ? the last remaining symbol of inter-Korean rapprochement. But officials in Seoul say hundreds of workers traveled as usual across the heavily armed border to the North Korean factory Monday as they have throughout the rising tensions.

"I have no idea about what it will be like when I go to the North Korean side. It seems OK to be here, but we will be living there in a tense situation for a week," Kim Won-soo, a South Korean manager working in Kaesong, said before his departure Monday from Paju, South Korea.

While analysts call North Korea's threats largely brinkmanship, there is some fear that a localized skirmish might escalate. Seoul has vowed to respond harshly should North Korea provoke its military. Naval skirmishes in disputed Yellow Sea waters off the Korean coast have led to bloody battles several times over the years. Attacks blamed on Pyongyang in 2010 killed 50 South Koreans.

South Korea's new president, Park Geun-hye, is pursuing a policy that seeks to re-engage North Korea with dialogue and aid after five years of standoff. But she told her military Monday to set aside political considerations and respond strongly should North Korea attack.

Meanwhile, deputies to North Korea's Supreme People's Assembly gathered in Pyongyang. The SPA schedule Monday was unclear.

Under late leader Kim Jong Il, North Korea had typically held a parliamentary meeting once a year. But Kim Jong Un held an unusual second session last September in a sign that he is trying to run the country differently from his father, who died in late 2011.

Parliament sessions, which usually are held to approve personnel changes and budget and fiscal plans, are scrutinized by the outside world for signs of key changes in policy and leadership.

At a session last April, Kim was made first chairman of the powerful National Defense Commission, the body's top post.

On Sunday, Kim presided over a separate plenary meeting of the Central Committee of the ruling Workers' Party, which set a "new strategic line" calling for building both a stronger economy and nuclear arsenal.

North Korea's nuclear weapons are a "treasure of a reunified country" not to be traded for "billions of dollars," according to a statement issued by state media after the meeting. North Korea's "nuclear armed forces represent the nation's life, which can never be abandoned as long as the imperialists and nuclear threats exist on earth."

Sunday marked the first time for Kim to preside over the committee meeting, a top decision-making body tasked with organizing and guiding the party's major projects. The last plenary session was held in 2010, according to Seoul's Unification Ministry, and before that in 1993.

The plenary statement also called for strengthening the moribund economy, which Kim has put an emphasis on in his public statements since taking power. The U.N. says two-thirds of the country's 24 million people face regular food shortages.

The North also named former Prime Minister Pak Pong Ju as a member of the party central committee's powerful Political Bureau, a sign that he could again play a key role in the North's economic policymaking process. Pak reportedly was sacked as premier in 2007 after proposing a wage system seen as too similar to U.S.-style capitalism.

Pak is reform-minded and his promotion sets him up for further advancement and "for him to take the lead in the North's economic policies," said Cheong Seong-jang at South Korea's Sejong Institute.

___

Follow Foster Klug at www.twitter.com/APKlug.

Source: http://news.yahoo.com/nkoreas-parliament-meets-amid-nuclear-tension-045319187.html

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